Promoter is the most important factor in transgenic. In this study effectivity of β-actine of medaka (Oryzias latipes) was examined in the eggs of walking catfish at the first cleavage and two cell stage. The experiment was carried out in the laboartory of Fish Genetic, Fac. Of Fisheries, Bogor Agricultural University for three month. Promooter was inserted by injecting artificial fertilization eggs with DNA pmβ-actin–hrGFP plasmid. DNA concentration was 20 µg/ml. Eggs development were observed at 8, 12, 16, 20, 24 hours after injection, 4 and 8 hours after hatching. The result showed that β-actin promoter was active on embryo targets indicated by expression of hrGFP gene after 8, 12, 16, 20, 24 hours after injection as well as 4 and 8 hours after hatching. Survival rate at 24 hours were 97,1% for control and 85,7% for injected eggs. Succesful of injection was higher on the first cleavage stage (66,7%) than two stage cell (50,0%). Hatching rate was also higher on first cleavage (93,3%) than two cell stage (55,0%). Total number of eggs injected succesfully was 35 with 57,1% of them containing foreign gene.
MH. Fariduddin Ath-thar, Komar Sumantadinata, Alimuddin dan Rudhy Gustiano
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