04 Februari 2009

EFFECTIVITY OF TANINE SOLUTION IN SUPPORTING THE SUCCESFULL OF EGGS MICROINJECTION ON AFRICAN CATFISH (Clarias gariepinus)

In gene transfer activity through microinjection, we need individual egg. Generally, the individual egg is resulted by removing the sticky layer covering egg surface. Among available solution, tanine is one alternative enabled to use for the eggs of African catfish. In this study, the effective dosage of tanine solution to remove the sticky layer of eggs was observed. The study was conducted in the Laboratory of Fish Breeding and Genetics, Bogor Agricultural University. Prior to microinjection, the eggs were immediately treated with different dosage of tanine solution after fertilization. There were four different level of dosage used as treatment, 0.3, 0.5, 0.7, and 0.9 ppm. After the treatment of tanine solution, the eggs were injected using DNA pmβ-actin-hrGFP plasmid with concentration of 20 μg/ml. The results showed that the dosage of 0.5 ppm tanine was the most effective treatment compare to the other dosages. This dosage produced 71.4% hatching rate and 57.1% transgenic embryos respectively.


By M.H. Fariduddin Ath-thar, K. Sumantadinata, Alimuddin, and R. Gustiano

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